Methods The last two abdomen segments of mosquitoes were cut down and treated with KOH, alcohol and xylene. Then the samples were observed under Nikon light microscope after sealed with neutral balsam on slides. 方法自雌蚊腹部剪下腹节最后两节,经KOH浸泡过夜后,酒精脱水、二甲苯透明,加拿大树胶封片后显微镜下观察。